Journal: International Journal of Molecular Sciences

Article Title: Theaflavin-3,3′-Digallate Enhances the Inhibitory Effect of Cisplatin by Regulating the Copper Transporter 1 and Glutathione in Human Ovarian Cancer Cells

doi: 10.3390/ijms19010117

Figure Lengend Snippet: Treatment with TF3 potentiated inhibitory effect of cisplatin against ovarian cancer A2780/CP70 and OVCAR3 cells via upregulating CTR1 protein expression. ( A ) The effect of TF3 at the designated concentrations on the protein levels of MRP2, ATP7A, ATP7B and CTR1 in ovarian cancer cells; ( B ) treatment with 7.5 μM TF3 upregulated CTR1 protein levels in ovarian cancer cells pretreated with 7.5 μM cisplatin; ( C ) transfection with CTR1 siRNA decreased CTR1 protein levels in ovarian cancer cells; ( D ) transfection with CTR1 siRNA enhanced the resistance of ovarian cancer cells to 7.5 μM cisplatin. Results are expressed as mean ± SD from three independent experiments. Significant differences among different treatments are marked with different letters ( p < 0.05), * ( p < 0.05) and ** ( p < 0.01).

Article Snippet: CTR1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (Danvers, MA, USA).

Techniques: Expressing, Transfection

Journal: PLoS ONE

Article Title: EGCG Enhances Cisplatin Sensitivity by Regulating Expression of the Copper and Cisplatin Influx Transporter CTR1 in Ovary Cancer

doi: 10.1371/journal.pone.0125402

Figure Lengend Snippet: (A) Effect of EGCG on mRNA expression of CTR1, CTR2, ATP7A and ATP7B. After OVCAR3 cells were treated with indicated concentrations of EGCG for 24h, RT-PCR and qPCR were carried out to measure the CTR1 mRNA expression. (B and C) Effect of EGCG on CTR1 protein expression in OVCAR3 and SKOV3 cells. The cells were treated with 10 μM EGCG for 24 h, then followed by western blot analysis. The bands were quantified with Image J software. (* P <0.05, ** P <0.01)

Article Snippet: Human CTR1 siRNA or siRNA control (RiboBio, Guangzhou, China) were transfected into OVCAR3 and HEK-293T cells in a 96-well plate using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions.Si-h-CTR1 sequences are listed below: si-h-CTR1 001: 5’ GAUCAAUACAGCUGGAGAA dTdT 3’ (forward) 3’dTdT CUAGUUAUGUCGACCUCUU 5’ (reverse); si-h-CTR1 002: 5’GGAAGAAGGCAGUGGUAGU dTdT 3’ (forward) 3’ dTdT CCUUCUUCCGUCACCAUCA 5’ (reverse); si-h-CTR1 003: 5’ CUACUUUGGCUUUAAGAAU dTdT 3’ (forward) 3’dTdT GAUGAAACCGAAAUUCUUA 5’ (reverse).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Software

Journal: PLoS ONE

Article Title: EGCG Enhances Cisplatin Sensitivity by Regulating Expression of the Copper and Cisplatin Influx Transporter CTR1 in Ovary Cancer

doi: 10.1371/journal.pone.0125402

Figure Lengend Snippet: (A) Effect of knock-down of CTR1 on the sensitivity of ovary cancer cells to cDDP. OVCAR3 cells were transfected with three company sythetic si-RNAs or siRNA control and the western blost analysis showed si-RNA 3 exhibiting the best effect. After transfected with si-RNA3 or siRNA control, OVCAR3 and SKOV3 cells were treated with cDDP at various doses for 48 h and the cell survival fraction was detected by MTT assay. (B) Embryonic kidney HEK-293 cells were tranfected with human CTR1 si-RNA3 or siRNA control. Then the cells were exposed by indicated doses of cDDP for 48 h and the cell survival fraction was detected by MTT assay. (* P <0.05).

Article Snippet: Human CTR1 siRNA or siRNA control (RiboBio, Guangzhou, China) were transfected into OVCAR3 and HEK-293T cells in a 96-well plate using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions.Si-h-CTR1 sequences are listed below: si-h-CTR1 001: 5’ GAUCAAUACAGCUGGAGAA dTdT 3’ (forward) 3’dTdT CUAGUUAUGUCGACCUCUU 5’ (reverse); si-h-CTR1 002: 5’GGAAGAAGGCAGUGGUAGU dTdT 3’ (forward) 3’ dTdT CCUUCUUCCGUCACCAUCA 5’ (reverse); si-h-CTR1 003: 5’ CUACUUUGGCUUUAAGAAU dTdT 3’ (forward) 3’dTdT GAUGAAACCGAAAUUCUUA 5’ (reverse).

Techniques: Knockdown, Transfection, Control, Western Blot, MTT Assay

Journal: PLoS ONE

Article Title: EGCG Enhances Cisplatin Sensitivity by Regulating Expression of the Copper and Cisplatin Influx Transporter CTR1 in Ovary Cancer

doi: 10.1371/journal.pone.0125402

Figure Lengend Snippet: (A, B) The effect of cDDP on the expression of CTR1 in OVCAR3 and SKOV3 cells. The cells were treated with 10 μM cDDP for the indicated time and CTR1 protein expression were detected by western blot analysis. (C)The effect of MG132 on the degradation of CTR1. After OVCAR3 cells were pretreated with 5 μM MG132 for 10h, the cells were incubation with 10 μM cDDP for 14 h. Then followed by western blot analysis. (D, E) The effect of EGCG on cDDP-trigged decrease of CTR1. The OVCAR3 and SKOV3 cells were treated with/without 10μM EGCG in the presence/absence of 10μMcDDP for 24 h. CTR1 protein expression was detected. The bands were quantified with Image J software. (* P <0.05, ** P <0.01)

Article Snippet: Human CTR1 siRNA or siRNA control (RiboBio, Guangzhou, China) were transfected into OVCAR3 and HEK-293T cells in a 96-well plate using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions.Si-h-CTR1 sequences are listed below: si-h-CTR1 001: 5’ GAUCAAUACAGCUGGAGAA dTdT 3’ (forward) 3’dTdT CUAGUUAUGUCGACCUCUU 5’ (reverse); si-h-CTR1 002: 5’GGAAGAAGGCAGUGGUAGU dTdT 3’ (forward) 3’ dTdT CCUUCUUCCGUCACCAUCA 5’ (reverse); si-h-CTR1 003: 5’ CUACUUUGGCUUUAAGAAU dTdT 3’ (forward) 3’dTdT GAUGAAACCGAAAUUCUUA 5’ (reverse).

Techniques: Expressing, Western Blot, Incubation, Software

Journal: PLoS ONE

Article Title: EGCG Enhances Cisplatin Sensitivity by Regulating Expression of the Copper and Cisplatin Influx Transporter CTR1 in Ovary Cancer

doi: 10.1371/journal.pone.0125402

Figure Lengend Snippet: Four groups (control, EGCG, cDDP and EGCG+cDDP) were set up. Except there were 6 mice in control group, there were 8 mice for each of the other groups. The body weight (A) and the tumor size (A) were measured twice a week. (B) The mRNA expression of the CTR1 in tumor tissues was measured by RT-PCR and real qPCR. (C) The expression of CTR1 in tumor tissue was assessed by western blotting. (D) The expression of CTR1 in kidney tissue was measured by western blotting. The bands were quantified by Image J software. (*P<0.05, **P<0.01)

Article Snippet: Human CTR1 siRNA or siRNA control (RiboBio, Guangzhou, China) were transfected into OVCAR3 and HEK-293T cells in a 96-well plate using Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions.Si-h-CTR1 sequences are listed below: si-h-CTR1 001: 5’ GAUCAAUACAGCUGGAGAA dTdT 3’ (forward) 3’dTdT CUAGUUAUGUCGACCUCUU 5’ (reverse); si-h-CTR1 002: 5’GGAAGAAGGCAGUGGUAGU dTdT 3’ (forward) 3’ dTdT CCUUCUUCCGUCACCAUCA 5’ (reverse); si-h-CTR1 003: 5’ CUACUUUGGCUUUAAGAAU dTdT 3’ (forward) 3’dTdT GAUGAAACCGAAAUUCUUA 5’ (reverse).

Techniques: Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Software

Journal: Oncotarget

Article Title: Dextran-Catechin: An anticancer chemically-modified natural compound targeting copper that attenuates neuroblastoma growth.

doi: 10.18632/oncotarget.10201

Figure Lengend Snippet: Figure 2: Levels of CTR1 protein expression and intracellular copper concentrations vary between neuroblastoma cell lines. (A) Representative Western blot for CTR1 protein on whole-cell extracts from IMR-32, IMR-32-CisRes, BE(2)-C, and MRC-5 cells. GAPDH expression was used as a protein loading control. (B) Densitometry graph of Western blots showing higher expression of CTR1 in IMR-32 and BE(2)-C cells compared to IMR-32-CisRes and normal MRC-5 cells. Values are normalized to GAPDH protein expression and shown relative to CTR1 expression in IMR-32 cells (100%). (C) Intracellular copper levels are higher in IMR-32 and BE(2)-C cells compared to IMR-32-CisRes and MRC-5 cells. Columns, means of at least three independent experiments; Bars, SEM (*P < 0.05, **P < 0.01).

Article Snippet: Twelve hours post-transfection with two different CTR1 specific siRNAs (siRNA A and siRNA B Origene, Rockville, MD, USA) or scrambled non-silencing siRNA, cells were treated with Dextran-Catechin for 24 hours.

Techniques: Expressing, Western Blot, Control

Journal: Oncotarget

Article Title: Dextran-Catechin: An anticancer chemically-modified natural compound targeting copper that attenuates neuroblastoma growth.

doi: 10.18632/oncotarget.10201

Figure Lengend Snippet: Figure 3: Knockdown of CTR1 in IMR-32 and BE(2)-C cells significantly reduced their sensitivity to Dextran-Catechin. (A) Cell death in IMR-32 and (B) BE(2)-C cells after knockdown of CTR1 and subsequent treatment with Dextran-Catechin for 24 hours. Columns, means of at least three independent experiments; Bars, SEM (***p < 0.001, ****p < 0.0001).

Article Snippet: Twelve hours post-transfection with two different CTR1 specific siRNAs (siRNA A and siRNA B Origene, Rockville, MD, USA) or scrambled non-silencing siRNA, cells were treated with Dextran-Catechin for 24 hours.

Techniques: Knockdown

Journal: bioRxiv

Article Title: Copper deficiency disrupts OXPHOS and mitochondrial dynamics through MTCH2-dependent copper trafficking in skeletal muscle

doi: 10.1101/2025.11.19.688750

Figure Lengend Snippet: a, Representative images of skeletal muscle from Floxed and SMKO mice. b, Immunofluorescence staining of myofiber types in the GA muscles of Floxed and SMKO mice. Scale bars = 100 μm. c, mRNA expression of slow- and fast-twitch fiber-related genes in the GA muscle of Floxed and SMKO mice ( n = 10). d, Cross-sectional area (CSA) of each myofiber in the GA muscle of Floxed and SMKO mice. CSA frequency was calculated using ImageJ. Scale bars = 100 μm. e, Energy expenditure of Floxed and SMKO mice. f, Oxygen consumption rate (OCR) of single myofibers isolated from the GA muscle of Floxed and SMKO mice following sequential treatment with oligomycin (Oligo), carbonyl cyanide p-triflouromethoxyphenylhydrazone (FCCP), rotenone (Rot), and antimycin A (AA). g, ATP levels in GA muscles of Floxed and SMKO mice ( n = 4). h, Extracellular acidification rate (ECAR) of C2C12 myoblasts after Ctr1 knockdown. siNC, negative control siRNA; si Ctr1 , Ctr1 siRNA i, Blood lactate concentration of Floxed and SMKO mice ( n = 6). Data are presented as mean ± SEM. Statistical differences were determined by two-way ANOVA with Šídák’s multiple comparisons test ( d-f , h ) and two-tailed Student’s t-test ( c , g , i ).

Article Snippet: C2C12 myoblasts were transfected with small interfering RNA targeting mouse Ctr1 , Mtch2 , or negative control (Bioneer) for 48 h using TransIT-X2® Transfection Reagent (Takara Bio Inc.) according to manufacturer’s instructions.

Techniques: Immunofluorescence, Staining, Muscles, Expressing, Isolation, Knockdown, Negative Control, Concentration Assay, Two Tailed Test

Journal: bioRxiv

Article Title: Copper deficiency disrupts OXPHOS and mitochondrial dynamics through MTCH2-dependent copper trafficking in skeletal muscle

doi: 10.1101/2025.11.19.688750

Figure Lengend Snippet: a-d , Proteomics analysis of the GA muscle of Floxed and SMKO mice. ( a ), Volcano plot. ( b ), GO analysis of DEPs. ( c ), STRING network analysis of DEPs. ( d ), GO analysis of downregulated proteins in SMKO compared to Floxed mice. e, Heatmap visualization of OXPHOS-related protein expression. f-h, Analysis of mitochondrial function in the GA muscle of Floxed and SMKO mice. ( f ), Protein levels of OXPHOS components and CCS. ( g ), SDH and modified Gomori trichorme (MGT) staining. Yellow arrows indicate ragged-red fibers. Scale bar = 100 μm. ( h ), Transmission electron microscopy (TEM) images. Scale bar = 2 μm (4,000× magnification), 1 μm (10,000× magnification). i, CTR1 expression levels of individuals with the MM compared to normal controls (extracted from GSE43698). Data are presented as mean ± SEM. Statistical differences were determined by two-tailed Student’s t-test ( i ).

Article Snippet: C2C12 myoblasts were transfected with small interfering RNA targeting mouse Ctr1 , Mtch2 , or negative control (Bioneer) for 48 h using TransIT-X2® Transfection Reagent (Takara Bio Inc.) according to manufacturer’s instructions.

Techniques: Expressing, Modification, Staining, Transmission Assay, Electron Microscopy, Two Tailed Test

Journal: bioRxiv

Article Title: Copper deficiency disrupts OXPHOS and mitochondrial dynamics through MTCH2-dependent copper trafficking in skeletal muscle

doi: 10.1101/2025.11.19.688750

Figure Lengend Snippet: A single intramuscular injection of AAV-DIO- Ctr1 was administered to SMKO mice, and assessments were performed after 5 weeks. a, Copper levels in the GA muscle ( n = 3). b, Running distance and duration in treadmill training ( n = 5). c, Immunofluorescence staining of myofiber types. d, OCR of single myofibers. e, Blood lactate concentration ( n = 4–5). f, Energy expenditure. g, OXPHOS, CCS, and MTCH2 protein levels, normalized to GAPDH expression. h, SDH and MGT staining. Scale bar = 100 μm. i, TEM images. Scale bar = 2 μm (4,000× magnification), 1 μm (10,000× magnification). Data are presented as mean ± SEM. Statistical differences were determined by one-way ANOVA ( a,b,e,g ) and two-way ANOVA with Tukey’s test ( c,d ) or Šídák’s multiple comparisons test ( f ). ns, not significant.

Article Snippet: C2C12 myoblasts were transfected with small interfering RNA targeting mouse Ctr1 , Mtch2 , or negative control (Bioneer) for 48 h using TransIT-X2® Transfection Reagent (Takara Bio Inc.) according to manufacturer’s instructions.

Techniques: Injection, Immunofluorescence, Staining, Concentration Assay, Expressing

Journal: Molecular Neurobiology

Article Title: Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders

doi: 10.1007/s12035-026-05692-4

Figure Lengend Snippet: TSPO elevation disrupts copper homeostasis in BV-2 cells. A Compare the changes in intracellular copper ion content among LPS-stimulated BV-2 cells across different groups. B Representative images of ATP7A, HSP70, CTR-1, FDX-1, and β-actin expression in LPS-stimulated BV-2 cells by western blot. C – F Comparison of ATP7A, HSP70, CTR-1, and FDX-1 expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Quantification of the fluorescence intensity of ATP7A using Image-Pro Plus ( n = 5). H Representative immunocytochemistry of ATP7A expression in the BV-2 cells. Scale bar, 20 µm. I Representative immunocytochemistry of CTR-1 expression in the BV-2 cells. Scale bar, 20 µm. J Representative immunocytochemistry of COX17 expression in the BV-2 cells. Scale bar, 20 µm. K Quantification of the fluorescence intensity of CTR-1 using Image-Pro Plus ( n = 5). L Quantification of the fluorescence intensity of COX17 using Image-Pro Plus ( n = 5). Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: CTR-1 Abmart , T510261 AB_3644314 , Rabbit 1:1000.

Techniques: Expressing, Western Blot, Comparison, Fluorescence, Immunocytochemistry

Journal: Molecular Neurobiology

Article Title: Mechanistic Investigation of TSPO-Mediated Dysregulation of Mitochondrial Copper Homeostasis in Microglia and its Role in Perioperative Neurocognitive Disorders

doi: 10.1007/s12035-026-05692-4

Figure Lengend Snippet: Disruption of copper homeostasis as a primary mechanism behind TSPO-induced mitochondrial structural and functional abnormalities. A , B , C Comparison of FDX-1 mRNA and protein expression in FDX-1 knockdown cells and the control siRNA cells ( n = 3). D Comparison of copper ion content in the LPS-stimulated BV-2 cells in each group ( n = 5). E Representative images of ATP7A and β-actin expression in LPS-stimulated BV-2 cells by western blot. F Comparison of ATP7A expression in the LPS-stimulated BV-2 cells in each group based on western blot analysis ( n = 5). G Comparison of CTR-1 expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). H Comparison of ATP7A expression in the LPS-stimulated BV-2 cells by group using qRT-PCR ( n = 5). I Quantification of the fluorescence intensity of the ROS using Image-Pro Plus ( n = 3). J Representative images showing the ROS expression in the LPS-stimulated BV-2 cells. Scale bar, 20 µm. K TEM examined the ultrastructure of cells, × 10,000. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: CTR-1 Abmart , T510261 AB_3644314 , Rabbit 1:1000.

Techniques: Disruption, Functional Assay, Comparison, Expressing, Knockdown, Control, Western Blot, Quantitative RT-PCR, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: Log β and pK values for Cu 2+ complexes with Ctr1 (1-14) at I = 0.1 mol dm −3 (KNO 3 ) and T = 298 K.

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: Species distribution for the Cu 2+ - Ctr1 (1-14) system. C Ctr1 = 5 × 10 −4 mol dm −3 ; Cu 2+ /Ctr1 (1-14) concentration ratio is 0.9, 1.2, 1.6 and 1.9 for ( a – d ), respectively.

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: Total concentration of Cu 2+ -Ctr1 (1-14) mononuclear and binuclear complexes species at Cu 2+ /Ctr1 (1-14) concentration ratios equal to 0.9, 1.2, 1.6 and 1.9 for ( a – d ), respectively.

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: Spectroscopic parameters obtained from the titration of the Cu 2+ -Ctr1 (1-14) .

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques: Titration

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: ( a ) Ascorbate-dependent reduction of Cu 2+ in the presence or absence of Ctr1 (1-14) at different metal/peptide ratios. Peptide (25 μM) and ascorbate (100 μM) solutions in MOPS (10 mM) at pH 7.2 were monitored for 30 min by UV-vis spectra at 265 nm. Change of ascorbate absorbance band: ascorbate without (black line) and with Cu 2+ (red line), 1:1 Cu 2+ /Ctr1 (1-14) ratio (blue), 2:1 Cu 2+ /Ctr1 (1-14) ratio (magenta), 3:1 Cu 2+ /Ctr1 (1-14) ratio (green). ( b ) UV-vis spectra of Cu 2+ -Ctr1 (1-14) system carried out in the same experimental condition of Cu 2+ ascorbate-dependent reduction; the brown line represents the difference spectra.

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: Species distribution for the Cu 2+ -Ctr1 (1-14) system. C Ctr1 = 2.5 × 10 −5 mol dm −3 ; Cu 2+ /Ctr1 (1-14) concentration ratio is 1.9.

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: Log β values obtained for the Ag + -Ctr1 (1-14) system at I = 0.1 mol dm −3 (KNO 3 ) and T = 298 K.

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: Species distribution of Ag + complexes with Ctr1 (1-14) (L). Ag + /Ctr1 (1-14) = 0.9; the analytical concentration of Ctr1 (1-14) is 5 × 10 −4 mol dm −3 .

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques: Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: ESR spectra (150 K) of the complex species of Ctr1 (1-14) , with Cu 2+ as function of the pH and the ligand-to-metal ratio (left panel, L:Cu = 1:1; right panel, L:Cu = 1:2). Computed spectra are shown in red.

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: ESR parallel Hamiltonian parameters of Cu 2+ with Ctr1 (1-14) at different metal-to-ligand ratios as a function of the pH.

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Deeper Insight in Metal Binding to the hCtr1 N-terminus Fragment: Affinity, Speciation and Binding Mode of Binuclear Cu 2+ and Mononuclear Ag + Complex Species

doi: 10.3390/ijms23062929

Figure Lengend Snippet: Immunoblots of Ctr1 (1-14) and Ctr1 (1-25) peptides (1 mM) pre-incubated with different concentrations of copper (L/Cu 2+ ratios 1:1, 1:2, 1:3) with or without 1% SDS. The membrane was incubated with anti-Ctr1 extracellular domain antibody.

Article Snippet: Ctr1 (1-14) and Ctr1 (1-25) were supplied by Caslo Aps, Lyngby, Denmark.

Techniques: Western Blot, Incubation, Membrane

Journal: Frontiers in Molecular Biosciences

Article Title: Multinuclear Metal-Binding Ability of the N-Terminal Region of Human Copper Transporter Ctr1: Dependence Upon pH and Metal Oxidation State

doi: 10.3389/fmolb.2022.897621

Figure Lengend Snippet: Stoichiometry of Ctr1 1-14 complexes with Cu(II) and Ag(I) ions observed by ESI-MS.

Article Snippet: The 14mer Ctr1 1-14 peptide (sequence MDHSHHMGMSYMDS, MM: 1665.85 Da; pI: 5.68) was purchased from Genscript Biotech (Piscataway, New Jersey, United States) and used without further purification.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Multinuclear Metal-Binding Ability of the N-Terminal Region of Human Copper Transporter Ctr1: Dependence Upon pH and Metal Oxidation State

doi: 10.3389/fmolb.2022.897621

Figure Lengend Snippet: (A) Superposition of 1D 1 H NMR spectra of Ctr1 1-14 in 25 mM Pi buffer at pH 7.0 ( blue contours ) and 3.5 ( red contours ). The regions enclosed in dashed boxes are expanded in (B) . The proton assignment scheme is shown in (C) where the structure of aminoacid side chains is reported.

Article Snippet: The 14mer Ctr1 1-14 peptide (sequence MDHSHHMGMSYMDS, MM: 1665.85 Da; pI: 5.68) was purchased from Genscript Biotech (Piscataway, New Jersey, United States) and used without further purification.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Multinuclear Metal-Binding Ability of the N-Terminal Region of Human Copper Transporter Ctr1: Dependence Upon pH and Metal Oxidation State

doi: 10.3389/fmolb.2022.897621

Figure Lengend Snippet: Overlay of three regions of 1D 1 H NMR spectra of Ctr1 1-14 in 10 mM MES (pH 7) at different Cu(II):peptide ratios. Proton assignment scheme as shown in .

Article Snippet: The 14mer Ctr1 1-14 peptide (sequence MDHSHHMGMSYMDS, MM: 1665.85 Da; pI: 5.68) was purchased from Genscript Biotech (Piscataway, New Jersey, United States) and used without further purification.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Multinuclear Metal-Binding Ability of the N-Terminal Region of Human Copper Transporter Ctr1: Dependence Upon pH and Metal Oxidation State

doi: 10.3389/fmolb.2022.897621

Figure Lengend Snippet: ESI-MS spectra of Ctr1 1-14 treated with 1 and 2 eq of CuSO 4 (A,C) and magnification of the doubly charged peaks ( cyan and blue dashed boxes ) displayed in (B,D) , respectively; the insets show the calculated isotopic distributions. (E) Intensity of doubly charged ESI-MS peaks of different Ctr1 1-14 species as a function of Cu(II):peptide ratio.

Article Snippet: The 14mer Ctr1 1-14 peptide (sequence MDHSHHMGMSYMDS, MM: 1665.85 Da; pI: 5.68) was purchased from Genscript Biotech (Piscataway, New Jersey, United States) and used without further purification.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Multinuclear Metal-Binding Ability of the N-Terminal Region of Human Copper Transporter Ctr1: Dependence Upon pH and Metal Oxidation State

doi: 10.3389/fmolb.2022.897621

Figure Lengend Snippet: Overlay of three regions of 1D 1 H NMR spectra of Ctr1 1-14 in 10 mM MES (pH 7) at different Ag(I):peptide ratios. Proton assignment scheme as shown in .

Article Snippet: The 14mer Ctr1 1-14 peptide (sequence MDHSHHMGMSYMDS, MM: 1665.85 Da; pI: 5.68) was purchased from Genscript Biotech (Piscataway, New Jersey, United States) and used without further purification.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Multinuclear Metal-Binding Ability of the N-Terminal Region of Human Copper Transporter Ctr1: Dependence Upon pH and Metal Oxidation State

doi: 10.3389/fmolb.2022.897621

Figure Lengend Snippet: ESI-MS spectra of Ctr1 1-14 treated with 1 and 2 eq of AgNO 3 (A,C) and magnification of the doubly charged peaks ( yellow and green dashed boxes ) displayed in (B,D) , respectively; the insets show the calculated isotopic distributions. (E) Intensity of doubly charged ESI-MS peaks of different Ctr1 1-14 species as a function of Ag(I):peptide ratio.

Article Snippet: The 14mer Ctr1 1-14 peptide (sequence MDHSHHMGMSYMDS, MM: 1665.85 Da; pI: 5.68) was purchased from Genscript Biotech (Piscataway, New Jersey, United States) and used without further purification.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Multinuclear Metal-Binding Ability of the N-Terminal Region of Human Copper Transporter Ctr1: Dependence Upon pH and Metal Oxidation State

doi: 10.3389/fmolb.2022.897621

Figure Lengend Snippet: Optimized structural models 1–4 (A–D) predicted in the PBE/DGDZVP framework for Ag(I)-Ctr1 1-14 . The silver ion is shown in green, oxygen in red, nitrogen in blue, carbon in gray and sulfur in yellow.

Article Snippet: The 14mer Ctr1 1-14 peptide (sequence MDHSHHMGMSYMDS, MM: 1665.85 Da; pI: 5.68) was purchased from Genscript Biotech (Piscataway, New Jersey, United States) and used without further purification.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Multinuclear Metal-Binding Ability of the N-Terminal Region of Human Copper Transporter Ctr1: Dependence Upon pH and Metal Oxidation State

doi: 10.3389/fmolb.2022.897621

Figure Lengend Snippet: (A) Overlay of three regions of 1D 1 H NMR spectra of Ctr1 1-14 containing 1 eq of Ag(I) in 10 mM MES (pH 7) treated with Cu(II) at different copper:peptide ratios; the dashed lines indicate the positions of the four methyl signals of methionines (He) before addition of Cu(II). Proton assignment scheme as shown in . (B) ESI-MS spectra of Ctr1 1-14 after treatment with 1 eq of AgNO 3 and 1 eq of CuSO 4 and (C) magnification of the doubly charged peak ( orange dashed box in B ); the inset shows the calculated isotope distribution.

Article Snippet: The 14mer Ctr1 1-14 peptide (sequence MDHSHHMGMSYMDS, MM: 1665.85 Da; pI: 5.68) was purchased from Genscript Biotech (Piscataway, New Jersey, United States) and used without further purification.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Multinuclear Metal-Binding Ability of the N-Terminal Region of Human Copper Transporter Ctr1: Dependence Upon pH and Metal Oxidation State

doi: 10.3389/fmolb.2022.897621

Figure Lengend Snippet: (A) Overlay of three regions of 1D 1 H NMR spectra of Ctr1 1-14 containing 1 eq of Cu(II) in 25 mM Pi buffer at different pH values, with or without ascorbate; the dashed lines indicate the positions of the corresponding proton signals at pH 3.5 in the absence of ascorbate. (B) Overlay of three regions of 1D 1 H NMR spectra of Ctr1 1-14 containing 2 eq of Cu(II) in 10 mM MES at different pH values, with or without ascorbate; the dashed lines indicate the positions of the corresponding proton signals at pH 7.0 in the absence of Cu(II). (C) Overlay of three regions of 1D 1 H NMR spectra of Ctr1 1-14 in 25 mM Pi buffer (pH 3.5) at different Ag(I):peptide ratios; the dashed lines indicate the positions of the corresponding proton signals in the absence of Ag(I).

Article Snippet: The 14mer Ctr1 1-14 peptide (sequence MDHSHHMGMSYMDS, MM: 1665.85 Da; pI: 5.68) was purchased from Genscript Biotech (Piscataway, New Jersey, United States) and used without further purification.

Techniques: